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EDTA dipotassium salt is a very important anticoagulant in vacuum blood tubes. EDTA has a great affinity for calcium ions in blood, and can complex with calcium ions, so that prothrombin can not be converted into thrombin, so that blood anticoagulation.
Introduction: EDTA dipotassium, cas 25102 12 9, is a kind of amino polycarboxylic acid and calcium chelating agent. As an anti-coagulation tube additive, it can effectively prevent blood samples from coagulating. It is suitable for general hematology test. It can also complex some ions in plasma to make some protein or nucleic acid substances more stable.
Detailed Introduction of EDTA Dipotassium
As an additive of vacuum blood tube, EDTA dipotassium can protect the cell composition of blood, not affect the count and size of white blood cell, has the least effect on the morphology of red blood cell, and can inhibit the aggregation of platelet, and is suitable for general hematology test. In addition, EDTA dipotassium can be used as a chelating agent in toilet deodorants and cosmetics, as a complexing agent in liquid phase analysis, and as a metal polishing agent.
EDTA dipotassium product advantages:
As anticoagulant for blood tubes, EDTA dipotassium can dissolve good anticoagulant effect.
EDTA dipotassium has no effect on white blood cell count and size, and little effect on erythrocyte morphology.
EDTA dipotassium can inhibit platelet aggregation and is suitable for general hematological examination.
EDTA dipotassium is considered as an ideal anticoagulant for whole blood count and classification.
Advantages of Desheng on EDTA Dipotassium:
The professional EDTA dipotassium R D technical team can solve all problems in time;
Desheng is a direct EDTA dipotassium salt manufacturer, with sufficient monthly output of 10t, which can meet the needs of general customers;
Decheng has many years of experience in R D and production of EDTA dipotassium. The purity can reach above 99.5% with good quality, and special indicators can be customized for production;
Have a professional foreign trade team with rich export experience. We have provided professional service to more than 100 customers.
Application scope of EDTA Dipotassium
EDTA dipotassium is usually added into the vacuum blood collection tube for blood routine examination Note: EDTA dipotassiumis not applicable to the coagulation test and platelet kinetic energy test, nor to the determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase and PCR test. In addition, EDTA dipotassium is generally used as the blood anticoagulant in the nucleic acid detection tube. EDTA dipotassium can also be used for complexing metal ions and separating metals, as well as for detergents, liquid soaps, shampoos, agricultural chemical sprays, antidotes, etc.
Technical Requirements for EDTA Dipotassium
No. Project Specifications
1 Content %? 99.0
2 PH 50g/L,25°C 4.8±1
3 Clarity test Qualified
4 Solubility %? 60
5 Chloride cl? 0.005
6 Sulfate SO4? 0.02
7 Iron Fe? 0.001
8 Heavy metal calculated as Pb? 0.001
Note:”%” in the table refers to mass fraction.
Cautions of EDTA dipotassium
In the whole blood sample collection test, the key step of preparation is that the blood should be fully contacted with EDTA dipotassium to achieve full anticoagulation. Therefore, it is necessary to gently reverse 58 times as soon as possible after blood collection to prevent the formation of tiny blood clots.
Although EDTA dipotassium is an anticoagulant, this kind of anticoagulation is non-reversible and the blood will still coagulate within a certain period of time, and the blood should be tested within 24 hours after anticoagulation with EDTA dipotassium, otherwise, the test results may be incorrect.
The preservation time of EDTA dipotassium anticoagulant samples was 48 hours at 4°C and less than 24 hours at 23°C, and 24 hours at 4°C.
Anticoagulation requires a sufficient amount of EDTA, but excessive EDTA may cause morphological changes in blood cells, and accurate control of EDTA dipotassium addition is particularly important.
EDTA dipotassium inhibits platelet aggregation, but does not completely prevent platelet aggregation.
EDTA dipotassium can be used in combination with various blood sampling additives, such as sodium fluoride, serum separation gel, etc., and the effect will be different with the use of different additives. For example, when EDTA dipotassium add sodium fluoride is used in the blood glucose test, adding 1.2-2.0mg dipotassium to each ml of blood, and 2-4mg sodium fluoride, while EDTA dipotassium serum separation gel can be used in the nucleic acid test tube.
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